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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Microvasc Res. 2015 Feb 7;98:126–138. doi: 10.1016/j.mvr.2015.01.006

Fig. 1. Molecular endothelial stem signature induced by ID3 overexpression.

Fig. 1

Cell surface expression of endothelial stem markers CD133, VEGFR3, CD34 was analyzed by two color flow cytometry. (A) Representative flow cytometry data analysis of EC wt and EC ID3+ analyzed for the co-expression of endothelial marker CD34 and stem marker CD133. (B) Representative flow cytometric analysis of CD34 and VEGFR3 co-expression in EC wt and EC ID3+. (C) CD133+ cells were approximately 7-fold higher in EC ID3+ compared to EC wt. Bar graph represents the flow cytometric data for CD133 and CD34 co-expression from three independent experiments ± SD. (D) VEGFR3+ cells were approximately 5-fold higher in EC ID3+ compared to EC wt. (E) Representative flow cytometry data analysis of EC wt and EC ID3+ analyzed for the co-expression of Sox2 and Oct4. (F) Representative flow cytometric analysis of CD31 and Pyk2 co-expression in EC wt and EC ID3+. (G) Sox2 and Oct4 positive cells were approximately 6.7-fold higher in EC ID3+ compared to EC wt. Bar graph represents the flow cytometric data for Sox2 and Oct4 co-expression from three independent experiments ± SD. (H) Pyk2 and CD31 positive cells were approximately 7-fold higher in EC ID3+ compared to EC wt. Graph of flow cytometric data for Pyk2 and CD31 co-expression derived from three independent experiments ± SD. Each column represents mean marker expression in either EC ID3+ **p<0.01 vs. EC wt for increase; or EC ID3+ b p<0.01 vs. EC wt for decrease. Data were analyzed by ANOVA; Tukey HSD test for multiple comparisons.