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. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Virology. 2015 Feb 7;478:135–142. doi: 10.1016/j.virol.2015.01.011

Transient HPV-31 replication was altered by modulating TopBP1 levels. (A) Western blot of TopBP1 in H1299 and C33A cells. Left panels show the western blot from H1299 and C33A cells transfected with pSUPER-siTopBP1 or its vector control. Right panel shows the western blot from H1299 cells transfected with vector or Flag-TopBP1 constructs. Actin was used as a loading control. Legend – V – vector controls, sh – pSUPER-siTopBP1, FT – Flag-TopBP1. (B) HPV-31 luciferase based assays in H1299 (upper) and C33A (lower) cells having either Knockdown or over-expression constructs of TopBP1. p < 0.05 between control and over-expression/knockdown at both time points for H1299, while it was significant at 72 hours for C33A.

Transient HPV-31 replication was altered by modulating TopBP1 levels. (A) Western blot of TopBP1 in H1299 and C33A cells. Left panels show the western blot from H1299 and C33A cells transfected with pSUPER-siTopBP1 or its vector control. Right panel shows the western blot from H1299 cells transfected with vector or Flag-TopBP1 constructs. Actin was used as a loading control. Legend – V – vector controls, sh – pSUPER-siTopBP1, FT – Flag-TopBP1. (B) HPV-31 luciferase based assays in H1299 (upper) and C33A (lower) cells having either Knockdown or over-expression constructs of TopBP1. p < 0.05 between control and over-expression/knockdown at both time points for H1299, while it was significant at 72 hours for C33A.