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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Gene. 2015 Feb 19;561(2):235–248. doi: 10.1016/j.gene.2015.02.050

Figure 10. Enhancer/Silencer function of SLC9B1 CpGII.

Figure 10

(A), (B) and (C). The SLC9B1 CpGII was cloned upstream of the promoters in the pGL3-hNHEDC1-promoter1 vector, pGL3-hNHEDC1-promoter2 and pCpGL-EF1α promoter vectors. The promoter only vectors along with CpGII-promoter reporter contructs and were transiently transfected into GC-1spg cells and HEK 293 cells along with pRL-TK vector. The reporter activity is shown as the ratio of firefly luminescence of the CpGII-promoter vectors to the corresponding promoter only vectors each normalized to the Renilla luciferase luminescence and depicted as fold change over the respective promoter only vector activity (set at 1.0). Bars indicate mean±SD three independent transfections performed in duplicate. (*) indicates statistically significant difference with p values < 0.05.