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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Gene. 2015 Feb 19;561(2):235–248. doi: 10.1016/j.gene.2015.02.050

Figure 11. Methylation dependence of silencer function of CpGII.

Figure 11

(A) & (C) The promoter only vectors (pGL3-hNHEDC1-promoter2 vector and pCpGL-EF1α promoter vector) and the pGL3-hNHEDC1-promoter2-CpGII and pCpGL-EF1α promoter-CpGII vectors were mock methylated (MM) and in vitro methylated (IV) methylated and transiently transfected along with the pRL-TK vector into HEK 293 and GC-1spg cells. The reporter activity is depicted as the normalized luciferase activity of the pGL3-hNHEDC1-promoter2-CpGII and pGL-EF1α promoter-CpGII MM and IV and depicted as fold change over the respective promoter only vector activity (set at 1.0 and indicated by open bars). (B) & (D) The pGL3-hNHEDC1-promoter1 vector and pGL3-hNHEDC1-promoter1-CpGII vector were MM and IV and transiently transfected along with pRL-TK vector into HEK 293 and GC-1spg cells. The reporter activity is depicted as fold change over the pGL3-hNHEDC1-promoter1 vector MM (set at 1.0). Bars indicate mean±SD of four independent transfections performed in duplicate. (*) indicates statistically significant difference from controls with p values < 0.05. Note: Prom1 and Prom2 indicate SLC9B1 promoter1 and promoter2 respectively.