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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Gene. 2015 Feb 19;561(2):235–248. doi: 10.1016/j.gene.2015.02.050

Figure 4. Analysis of the putative SLC9B1 promoter 2 activity in HEK 293 and GC-1spg cells.

Figure 4

The P2 was cloned upstream of the firefly luciferase to generate the pGL3-hNHEDC1-promoter2 vector and analyzed for luciferase expression in HEK 293 and GC-1spg cells. The reporter activity is shown as the ratio of firefly luminescence of the pGL3-hNHEDC1-promoter2 to the pGL3-Basic (empty vector) each normalized to the Renilla luciferase luminescence and depicted as fold increase over the promoterless pGL3-Basic vector activity (set at 1.0). Bars indicate mean±SD of three independent transfections performed in duplicate. (*) indicates statistically significant difference with p values < 0.05.