Figure 6. The SLC9B1 P1/CpGI but not CpGII shows differences in methylation in human testis and lung.
(A) The SLC9B1 P1/CpGI is hypomethylated in human testis and lung. The part of P1/CpGI with the maximum number of CpGs was used for bisulfite sequencing analysis by amplification of a single 450bp fragment. (B) The SLC9B1 CpGII is methylated in the human testis and lung. The SLC9B1 CpGII was amplified as a 527bp fragment which contains the maximum number of CpGs. Columns represent individual CpGs, rows represent individual clones. Open circles represent unmethylated CpGs and closed circles represent methylated CpGs. A total of nine clones were analyzed from one biological replicate.