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Biliary concentrations of bile acids and not serum concentrations cause toxicity in human hepatocytes. LDH release was measured in human hepatocytes after exposure to a complete serum BA milieu or a derivation of this concentration (A) or a complete biliary bile acid milieu or a derivation of this concentration for 6 or 24 h (B). N=3 batches of isolated cells;. *P<0.05 (versus control).
