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. 2015 Mar 20;22(9):731–743. doi: 10.1089/ars.2014.5998

FIG. 2.

FIG. 2.

SrxC84 is recycled by the GSH/Grx/GR system in the steady state. The reaction of 50 μM C171A Prx–SO2 with 10 μM SrxC84 in the presence of 1 mM MgCl2 was followed using the GSH/GR (0.5 μM GR and 200 μM NADPH, circles) or GSH/Grx/GR (50 μM Grx, 0.5 μM GR, and 200 μM NADPH, squares) coupled assays. The assay was started by the addition of 1 mM ATP. Initial rate measurements were carried out at 30°C in buffer TK by following the decrease of the absorbance at 340 nm due to the oxidation of NADPH. The observed steady-state rate constant kss was calculated as the ratio between the rate measured and Srx concentration.