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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cell Signal. 2015 Feb 12;27(5):908–922. doi: 10.1016/j.cellsig.2015.02.003

Figure 3. UCR1C activates endogenous PDE4 long isoforms.

Figure 3

HEK293T cells were transfected with cerulean-vector, cerulean-UCR1C, or cerulean-UCR1C R/A. Forty-eight hours later, cell lysates were harvested, and 1.5 mg total protein was applied for immunoprecipitation using protein A-agarose beads, and anti-PDE4B pan-antibody or anti-UCR1 antibody. Relative PDE activities of endogenous PDE4 long isoforms (anti-UCR1 IP) are shown in (A), and endogenous PDE4B (anti-PDE4B IP) in (B). The data were shown as mean± s.e.m from two independent experiments.