Fig. 5.

Measurements of a single isolated mitochondrion. (A) DIC image of a mitochondrion in the isotonic buffer when the direction of the rotary polarizer was 40°. (B) The changes in the light intensity upon rotating the rotary polarizer. Circles, triangles, and squares indicate the intensity at the corresponding regions shown in (A). The solid lines are curves obtained by fitting Eq. (1) to the data. (C) ΔOPL map of a mitochondrion. (D) Changes in the ΔOPL along a line through the center of the mitochondrion image (open circle). The solid line is the interpolation curve. (E) A transmitted light image of a mitochondrion. (F) The changes in the intensity of the transmitted light along a line through the center of the mitochondrion image (open circle). The solid line is the approximated curve obtained by fitting the Gaussian function to the data.