Figure 1.

Flow cytometric gating strategy. Within the PBMCs of a healthy subject, lymphocytes (a) were identified on the basis of forward scatter and side scatter. We then identified the viable population of lymphocytes (b). The CD3⁺ T cell component of the viable cells (c) was then divided into CD4⁺ T cells and CD8⁺ T cells (d). Finally the CD4⁺ T cells and CD8⁺ T cells were subdivided into naïve, central memory (CM), effector memory (EM) and effector memory re-expressing CD45RA (EMRA) subsets according to the expression of CD45RA and CD62L ((e) and (f), respectively).