Fig 3. IL-24 inhibited the signaling proteins downstream of CXCR4.
Western blotting showed induction of IL-24 protein expression in H1299-IL24 cells resulted in marked reduction in the expression of phosphorylated (p) AKTS473, pmTORS2448 and pPRAS40T246 and HIF-1α at 24 h and 48 h after doxycycline treatment. Beta actin was used as protein loading control. Differences in the expression of the proteins was determined by semi-quantitative analysis and represented in graphical format (P<0.05). Bars denote standard deviation (SD).