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. 2015 Mar 16;10(3):e0119030. doi: 10.1371/journal.pone.0119030

Fig 1. NF1 inactivation in the adrenal gland cortex.

Fig 1

Whole mount LacZ staining showing a mosaic Cre-mediated recombination in adrenal cortex (dotted line) of 5-weeks-old Rosa26-lacZ reporter x Prx1-Cre mice. (B) Similar mosaic Cre recombination in the adrenal cortex visualized with mT/mG reporter mice. Note, no recombination was observed in the medulla. (C) PCR-based detection of recombined allele in the genomic DNA of 2-month-old Nf1Prx1 animals with varying degree of inactivation in the individual adrenal glands. Knock-out efficiency in the individual Nf1Prx1 adrenal glands assessed by quantitative RT-PCR. Note, out of 12 analysed mutant adrenal glands only one showed less than 30% efficiency of NF1 inactivation. c—cortex, m—medulla, f- fat, L- liver.