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. 2015 Mar 16;10(3):e0120896. doi: 10.1371/journal.pone.0120896

Fig 4. Exogenous NAD+ interrupts GAPDH-telomeric association in epimastigote cells.

Fig 4

(A) and (B) T. cruzi epimastigote cells were maintained in the presence of NAD+ or NADH for 10 min. Afterwards, cells were lysed and the amount of intracellular NAD+ and NADH was quantified. Graph shows mean and standard deviation of five independent experiments. Statistic analysis was performed using 2way ANOVA, * means p < 0.001. (C) The same samples were subjected to a chromatin immunoprecipitation assay using an anti-GAPDH antibody. After immunoprecipitation, cross-links were reversed, and DNA was extracted and immobilized onto a nylon membrane. The membranes were hybridized to a telomeric probe (CCCATT)6.