Table 1. Sense and antisense primers used in the assessment of mRNA expression for β-actin, CGRP, COX-1, COX-2 and TNF-α by reverse transcriptase polymerase chain reaction (RT-PCR).

GENE	PRIMER
β-actin	Sense: 5’-TTG TAA CCA ACT GGG ACG ATA TGG-3’Antisense: 5’-GAT CTT GAT CTT CAT GGT GCT AGG-3’
CGRP	Sense: 5′-AAG TTC TCC CCT TTC CTG GT-3′Antisense: 5′-GGT GGG CAC AAA GTT GTC CT-3′
COX-1	Sense: 5′-AGC CCC TCA TTC ACC CAT CAT TT-3′Antisense: 5′-CAG GGA CGC CTG TTC TAC GG- 3′
COX-2	Sense: 5’–ACA ACA TTC CCT TCC TTC-3’Antisense: 5’–CCT TAT TTC CTT TCA CAC C-3’
TNF-α	Sense: 5’-TAC TGA ACT TCG GGG TGA TTG GTC C-3’Antisense: 5’–CAG CCT TGT CCC TTG AAG AGA ACC-3’

Footnotes: CGRP—calcitonin gene-related peptide; COX—cyclooxygenase, TNF—tumor necrosis factor