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. 2015 Mar 16;11(3):e1004721. doi: 10.1371/journal.ppat.1004721

Fig 2. Tax interacts with CADM1.

Fig 2

(A) Jurkat T-cells were transduced with Tax-expressing lentiviruses. After 48 hours, cells were lysed and immunoprecipitated with either anti-Tax or control anti-IgG, followed by immunoblotting with anti-Tax and anti-CADM1 antibodies. Lysates were examined for Tax, CADM1, and β-actin expression. (B) Lysates from Tax expressing HTLV-1 transformed C8166, MT-2 and MT-4 cells were immunoprecipitated in duplicate with either anti-CADM1 or control anti-IgG, followed by immunoblotting with anti-Tax and anti-CADM1. Lysates were examined for Tax, CADM1, and β-actin expression. (C) A schematic overview of the FLAG-CADM1 deletion mutants ΔSP, ΔCP, ΔEC, ΔPDZ-BM and ΔFERM. (D) Mapping the interaction between CADM1 and Tax. Primary Cadm1 −/− MEFs were transfected with Tax expression vector with the indicated Flag-CADM1 mutants. After Thirty-six hours post-transfection, proteins from lysates were immunoprecipitated with anti-Flag and detected by immunoblotting with anti-Tax and anti-Flag antibodies. Lysates were immunoblotted with anti-Tax, and anti-Flag antibodies.