Fig 7. Tax requires CADM1 to inhibit IKKα-mediated phosphorylation of TAX₁BP₁ and inactivate the NF-κB inhibitor ubiquitin-editing enzyme A20 complex in the TNFR pathway.

Primary Cadm1 ^+/+ and Cadm1 ^−/− MEFs were transduced with Tax-expressing lentiviruses. After 48 hour, cells were treated for up to 120 minutes with TNF-α and lysates were immunoprecipitated with anti-TAX1BP1 followed by immunoblotting with anti-Tax, anti-Ubc13, anti-A20, anti-CADM1, anti-NEMO, and anti-TAX₁BP₁ antibodies. Lysates were examined for Tax, Ubc13, TAX₁BP₁, phospho-TAX₁BP₁, CADM1, NEMO, A20, TAK-1, phospho-TAK1, IκBα, phospho-IκBα, and β-actin expression.