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. 2015 Mar 16;10(3):e0120722. doi: 10.1371/journal.pone.0120722

Fig 5. Localization of SEPT12 and SPAG4 during human spermiogenesis.

Fig 5

IFA showed multiple localizations of SEPT12 and SPAG4 signals. A total of 32 male germ cells were evaluated as(A) Round spermatids (n = 7; labeled with anti-SEPT12 and anti-SPAG4 antibodies as the presented figure, 7/7); (B) elongating and (C) elongated spermatids (n = 12;12/12); (D) ejaculated spermatozoa (n = 13;12/13). (A–D) Anti-SEPT12 antibody (left panel; green), anti-SPAG4 antibody (middle panel; red), and a combination of the left and middle panels (right panel). The arrow indicates SEPT12 or SAPG4 signals. Scale bar: 5 μm.