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. 2015 Mar 16;10(3):e0120722. doi: 10.1371/journal.pone.0120722

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© 2015 Yeh et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — SEPT12-WT (wild-type), SEPT12^T89M (Thr89Met), SEPT12^D197N (Asp197Asn), and SEPT12^Del (c.474G/A-induced deleted form) are shown in Panels 1, 2, 3, and 4, respectively. Co-IP of FLAG-SPAG4 and LAIMINB1 with SEPT12-GFP. The cell lysates of the NT2D1 cells, transfected with pEGFP-SEPT12 or mutated SEPT12 and a pFLAG-SPAG4 vector, are subjected to IP with an anti-GFP antibody (Row 3) or a nonspecific control IgG (Row 2), followed by IB with an anti-GFP, anti-FLAG, or anti-LAMINB1 antibody. An input protein (5%) was used as a control during the IB of the transfected cell lysates (Row 1).