Fig 5. Changes in bone DMP1 expression with doxercalciferol therapy.

a) The quantification of DMP1 mRNA (qRT-PCR) transcript and DMP1 protein as detected by immunohistochemistry (IHC). For qRT-PCR, gene expression in dialysis patients before and after doxercalciferol therapy was expressed relative to normal control and was calculated as 2^(ΔCt DMP1)/2^ (ΔCt GAPDH). Individual data points are shown; the bar indicates the median value. The number sign indicates a significant (p<0.05) change from baseline. b) Western blot analysis of bone extract for DMP1 demonstrated higher amounts of 57kDa and lower amounts of 37kDa fragments in bone extracts of dialysis patients than of healthy control. The presence of the 57kDa fragment did not differ from control after treatment with doxercalciferol. Pts 1, 2, and 3 correspond to Pts 1, 2, and 3 in Fig. 3. Abbreviations: Ctr: healthy control; Pt: patient; ¥: p<0.05 from healthy control