Fig 2. Mutation of jhamt decreases JHB3 but not JH III and MF biosynthesis.

(A) Measurements of methyltransferase activity in the brain-RG complexes in w ¹¹¹⁸ and jhamt ² at 3hAIW using FA or JHA as the substrate. (B) Measurements of JH biosynthesis in the brain-RG complexes in w ¹¹¹⁸ and jhamt ² at 3hAIW using the RCA-TLC method. (C-C”) Quantitative measurements of whole body titers of JHB3 (C), JH III (C’), and MF (C”) in w ¹¹¹⁸ and jhamt ² at 3hAIW using the HPLC-FD protocol.