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. 2015 Mar 16;11(3):e1005038. doi: 10.1371/journal.pgen.1005038

Fig 5. MF plays a dual role: as a JHB3 precursor and as a hormone.

Fig 5

(A) Percentage of rescuing Aug21-GAL4>UAS-Grim, jhamt 2/jhamt 2 ; jhamt-GAL4>UAS-hmgcr dsRNA, and Met 27 gce 2.5k to adults by topical application of methoprene, JHB3, JH III and MF (0.5×10-2 μmol per larva) at 96h AEL. (B) Percentage of rescuing jhamt 2/jhamt 2 ; jhamt-GAL4>UAS-hmgcr dsRNA to adults by topical application of a dose gradient of methoprene, JHB3, JH III, and MF (0.5×10-9~-2 μmol per larva) at 96h AEL. (C) qPCR measurements of fold-changes of relative Kr-h1 mRNA levels in Kc cells treated with methoprene, JHB3, JH III, and MF (1×10-10~-6 M) for 30 min. (D) qPCR measurements of relative Kr-h1 mRNA levels in fat body tissues isolated from w 1118 and Met 27 gce 2.5k at 96h AEL after treatments with methoprene, JHB3, JH III, and MF (1×10-6 M) for 30 min. (E) qPCR measurements of the relative Kr-h1 mRNA levels in the fat body tissues isolated from w 1118, jhamt 2, Met 27, gce 2.5k, and Met 27 gce 2.5k at 3h AIW. (F) MF promotes interaction of Met and SRC in mouse embryonic fibroblast 3T3 cells. 3T3 cells were transiently transfected with GAL4:TcMet and TcSRC. And the transfected cells were cultured in the medium containing different concentrations of MF and JH III (DMSO as control). After 24 hours exposure to the ligands, cells were assayed for luciferase reporter activity. The luciferase activity was normalized based on the total protein concentration determined for cells in each well. (G-G”) Measurements whole body titers of JHB3 (G), JH III (G’), and MF (G”) in jhamt 2/jhamt 2 ; jhamt-GAL4>UAS-hmgcr dsRNA at 3hAIW after topical application of MF (0.5×10-2 μmol per larva; dissolved in acetone) at 96h AEL (about 24 hours after treatments).