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. 2015 Mar 16;10(3):e0119818. doi: 10.1371/journal.pone.0119818

Fig 6. Genotyping of the cell population in each step of mrfp replacement in four different loci.

Fig 6

Before, after, and during the 4 successive rounds of gene replacement, all 9 cultures were subjected to competitive PCR analysis using 3 primers annealing to the target, HKH cassette, and p T7-mrfp cassette sequences. The local sequence, location of the primers and expected size (in bp) of the PCR products are presented for the (1) yiiDE, (2) proV, (3) lacZ, and (4) rssB loci. The sequences of the primers used are shown in S1 Table.