Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 16;10(3):e0119874. doi: 10.1371/journal.pone.0119874

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Hoylaerts et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 6 — Dose-response of stimulation of AP activity (mean mA405nm/min) in steady-state (i.e. the slope measured between 60–90 min) at pH 9.8 (10 mM pNPP) for increasing [CaCl₂], added to AbM2-bound Zn²⁺-TNAP and the indicated mutants, after pre-treatment with EDTA (2 h) and loading with 20 μM Zn²⁺; correlation between calculated apparent K_ds for the functionally relevant Mg²⁺ and Ca²⁺ binding to TNAP and the three indicated TNAP mutants. Lines constructed in the absence and presence of 20 μM ZnCl₂ are as indicated; Apparent K_ds are represented with their respective SD.