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. 2015 Mar 16;10(3):e0120549. doi: 10.1371/journal.pone.0120549

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© 2015 Itagaki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — NET formation in PMN isolated from young healthy volunteers (n = 10, A and E), young trauma patients (n = 7, B and F), old healthy volunteers (n = 6, C and G), and old trauma patients (n = 5, D and H) was evaluated. A-D: PMN were immediately fixed after attachment. E-H: PMN were stimulated with 20 nM PMA for 3–4 h.