Table 2. Sequence of the primers used in the qPCR analysis.
| Target gene | Sequence ID of homologous genes in the B. floridae genome (JGI) | Primer sequences | Primer efficiency | BestKeeper standard deviation in quantification through development | BestKeeper standard deviation in quantification in the adult |
|---|---|---|---|---|---|
| NR1H-2 | Bf_124680 | Fwd: CGCCCTATTGTTATGGAAGC, Rev: AAGTGATGCGGTGTTCTGC | 1,961 | 0,66 | |
| NR1H-5 | Bf_128090 | Fwd: ATTTTGCCAGGCACGATG, Rev: CACTTGGCAAACTGAACGAG | 1,891 | 0,68 | |
| NR1H-6 | Bf_124948 | Fwd: TGCAGTGTGTGATAGTGGTGTC, Rev: GTAGGCGACTTTGCAGTAAGC | 1,878 | 0,65 | |
| NR1H-7 | Bf_156544 | Fwd: AGTCAAGGGTTCAGTTGTAGAGG, Rev: TATGGGCTGAGATGAGGATG | 1,928 | 0,49 | |
| NR1H-8 | Bf_222287 | Fwd: ACACCGCTTATGCAATCCTG, Rev: ATCGTCGTCTTGATGTACGC | 1,925 | 0,45 | |
| EF1 | Bf_58879 | Fwd: GGAAGTTCGAGACCACCAAG, Rev: CACAATCAGCACAGCACAGTC | 1,946 | 0,65 | |
| ILP | Bf_121099 | Fwd: TCGGCTCCTTCGAAGATAAG, Rev: CGTTGGTTGGTCCTTCTTCTAC | 1,877 | 2,1 | 1,87 |