Fig 3. Examination of the interaction of Rre3, CopR, NrsR and CcaR with Trx in E. coli cells.

Soluble proteins from the E. coli Origami2 strain (Control), the strain expressing only TrxM_C35S (Trx), the strain expressing only a TF and the strain expressing both TrxM_C35S and a TF were separated by non-reducing 12% SDS-PAGE and immunoblot analysis was performed. (A) Rre3, (B) CopR, (C) NrsR and (D) CcaR were detected using a His-tag antibody (left panel) and Trx was detected with S-protein (right panel). ± indicates with or without 100 mM DTT treatment. Black and white arrows indicate the TF monomer and the Trx monomer, respectively.