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. 2015 Mar 16;10(3):e0119107. doi: 10.1371/journal.pone.0119107

Fig 4. Examination of the interaction of Rre28, Rre37 and SphR with Trx in E. coli cells.

Fig 4

Soluble proteins from the E. coli Origami2 strain (Control), the strain expressing only TrxMC35S (Trx), the strain expressing only a TF and the strain expressing both TrxMC35S and a TF were separated by non-reducing 12% SDS-PAGE and immunoblot analysis was performed. (A) Rre28, (B) Rre37 and (C) SphR were detected using a His-tag antibody (left panel) and Trx was detected using S-protein (right panel). ± indicates with or without 100 mM DTT treatment. Black and white arrows indicate the TF monomer and the Trx monomer, respectively.