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. 2015 Mar 16;10(3):e0119107. doi: 10.1371/journal.pone.0119107

Table 1. Primers used in this study.

Primers Sequence
NdeI-Rre28-Fw AACATATGAGTCACCGTGTTCTA
XhoI-Rre28-Rv AACTCGAGATCCCTTAGTACGTAGCC
NdeI-Rre3-Fw AACATATGTGGGGGAACAGGACT
XhoI-Rre3-Rv AACTCGAGATCAGGGTCTTCAAACTT
NdeI-CopR-Fw AACATATGAGACTGTTGCTGGTG
XhoI-CopR-Rv AACTCGAGTTGTTCTGCATGGGTTGG
NdeI-NrsR-Fw AACATATGCGAATTTTGCTGGTG
XhoI-NrsR-Rv AACTCGAGTCATGGCGATAGGGTGAA
NdeI-Rre37-Fw AACATATGAATCCAGTCTACATA
XhoI-Rre37-Rv AACTCGAGGGTAAGTACAGAGACTCC
NdeI-SphR-Fw AACATATGTTGGTTAAATACCAT
XhoI-SphR-Rv AACTCGAGACCAAAACGATAGCCAAA
NdeI-RpaA-Fw AACATATGCCTCGAATACTGATC
XhoI-RpaA-Rv AACTCGAGCTACGTTGGACTACCGCC
NdeI-CcaR-Fw AACATATGAGAATTCTTTTAGTG
XhoI-CcaR-Rv AACTCGAGGTTTTTCCCTTGGCACAA
NdeI-ManR-Fw AACATATGGCCAATATCCTCTTA
XhoI-ManR-Rv AACTCGAGATTGGCCTCAAAACAGTA
NdeI-Trx-F AACATATGAGTGCTACCCCTCAA
XhoI-Trx-R AACTCGAGAAGATATTTTTCTAGGGT
Trx C35S-F AGTCGCATGGTAGCCCCCG
Trx C35S-R AGGGCCACACCAGGGAGC

Underlined letters indicate the restriction enzyme recognition sites, used for cloning purposes and bold letters indicate mutated bases to introduce cysteine to serine substitution in TrxM.