Table 1. Primers used in this study.
Primers | Sequence |
---|---|
NdeI-Rre28-Fw | AACATATGAGTCACCGTGTTCTA |
XhoI-Rre28-Rv | AACTCGAGATCCCTTAGTACGTAGCC |
NdeI-Rre3-Fw | AACATATGTGGGGGAACAGGACT |
XhoI-Rre3-Rv | AACTCGAGATCAGGGTCTTCAAACTT |
NdeI-CopR-Fw | AACATATGAGACTGTTGCTGGTG |
XhoI-CopR-Rv | AACTCGAGTTGTTCTGCATGGGTTGG |
NdeI-NrsR-Fw | AACATATGCGAATTTTGCTGGTG |
XhoI-NrsR-Rv | AACTCGAGTCATGGCGATAGGGTGAA |
NdeI-Rre37-Fw | AACATATGAATCCAGTCTACATA |
XhoI-Rre37-Rv | AACTCGAGGGTAAGTACAGAGACTCC |
NdeI-SphR-Fw | AACATATGTTGGTTAAATACCAT |
XhoI-SphR-Rv | AACTCGAGACCAAAACGATAGCCAAA |
NdeI-RpaA-Fw | AACATATGCCTCGAATACTGATC |
XhoI-RpaA-Rv | AACTCGAGCTACGTTGGACTACCGCC |
NdeI-CcaR-Fw | AACATATGAGAATTCTTTTAGTG |
XhoI-CcaR-Rv | AACTCGAGGTTTTTCCCTTGGCACAA |
NdeI-ManR-Fw | AACATATGGCCAATATCCTCTTA |
XhoI-ManR-Rv | AACTCGAGATTGGCCTCAAAACAGTA |
NdeI-Trx-F | AACATATGAGTGCTACCCCTCAA |
XhoI-Trx-R | AACTCGAGAAGATATTTTTCTAGGGT |
Trx C35S-F | AGTCGCATGGTAGCCCCCG |
Trx C35S-R | AGGGCCACACCAGGGAGC |
Underlined letters indicate the restriction enzyme recognition sites, used for cloning purposes and bold letters indicate mutated bases to introduce cysteine to serine substitution in TrxM.