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. 2015 Mar 16;10(3):e0116938. doi: 10.1371/journal.pone.0116938

Fig 2. Effect of H-Ras on exogenous ENaC expressed in FRT cells.

Fig 2

A, Immunoblot analysis detecting expression of H-Ras, upper panel, and corresponding Iami (normalized), lower panel, in FRT cells co-transfected with all three wild-type α-, β- and γ-ENaC subunits (ENaC) together with pcDNA3.1, wild-type H-Ras (wt-H-Ras), H-RasG12V or a constitutively inactive mutant of H-Ras (H-RasS17N). B, Iami (normalized) in FRT cells transfected with wild-type α-, β- and γ-ENaC together with empty pcDNA3.1 empty vector, K-RasG12V or H-RasG12V, and with or without the dominant negative mutant of PI3K, PI3KΔp85. In some experiments, cells were pre-treated with insulin (100 nM) for 2 hr. *, ** and *** denote P < 0.05, P < 0.01 and P < 0.001, respectively.