Table 3. Labeling intensity and median distance of label for Shank (pan Shank antibody) in the PSD complex under control and EGTA conditions.
| Labeling intensity (# label/μm PSD) Mean ± SEM (n = number of synaptic profiles measured) | Median distance (nm) from postsynaptic membrane (n = number of labels measured) | |||
|---|---|---|---|---|
| Control | EGTA | Control | EGTA | |
| Exp 1 | 17.9 ± 1.4 (23) | 12.0 ± 1.5 (17) P<0.01 | 57.8 (377) | 52.9 (334) P<0.05 |
| Exp 2 | 26.6 ± 2.1 (27) | 19.8 ± 1.4 (48) P<0.01 | 53.3 (164) | 40.0 (228) P<0.0001 |
| Exp 3 | 26.9 ± 1.5 (47) | 22.3 ± 1.3 (62) P<0.05 | 46.7 (227) | 45.0 (343) N.S. |
| Exp 4 | 24.7 ± 1.7 (65) | 14.7 ± 1.3 (51) P<0.0001 | 53.3 (311) | 36.7 (144) P<0.0001 |
| mean | 24.0 ± 2.1 | 17.3 ± 2.3 | 52.8 ± 2.3 | 43.7 ± 3.5 |
Statistical analysis: labeling intensity, Student’s t-test; median distance, Wilcoxon rank-sum test. N. S.: not significant.