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. 2014 Oct 8;290(2):471–484. doi: 10.1007/s00438-014-0931-4

Table 3.

Characterization of symbiosis between WT and miR156+ T2 L. japonicus plants

Time Morphology ‘Gifu’ WT miR156 + #20 miR156 + #22
7 dpi No. plants with infection threads 7 2 1
No. plants with nodule primordiai 2 0 0
14 dpi Root length (cm)

7.24 ± 0.31

(7.28 ± 0.31)

6.19 ± 0.30 *

(6.37 ± 0.25)*

5.85 ± 0.26 *

(6.08 ± 0.32)*
Root branch number

2.45 ± 0.26

(2.31 ± 0.26)

1.11 ± 0.19*

(1.25 ± 0.29)*

2.27 ± 0.20

(2.23 ± 0.23)
Nodule number 3.27 ± 0.29 1.67 ± 0.32 * 1.55 ± 0.26*
21 dpi Root length (cm)

10.49 ± 0.41

(11.57 ± 0.26)

9.64 ± 0.15 *

(10.57 ± 0.34)*

9.25 ± 0.46*

(10.8 ± 0.35*)
Root branch number

6.36 ± 0.34

(6.22 ± 0.57)

5.64 ± 0.41*

(5.1 ± 0.42)*

5.8 ± 0.55*

(7.56 ± 0.69)*
Nodule number 5.55 ± 0.25 3.82 ± 0.26 * 3.50 ± 0.31*
4 months old Root length (cm) 43.60 ± 1.86 33.40 ± 1.44* 35.20 ± 1.39*
Nodule number 432 ± 12.41 288 ± 19.85* 278 ± 17.15*

For 7, 14, and 21 dpi (14-, 21-, and 28-day-old seedlings, respectively) experiment, at least 10 seedlings were collected and measured. Values are mean ± SE. Numbers in brackets were derived from a non-bacterial inoculated control experiment. Root length and nodule numbers from five 4-month-old inoculated seedlings for each line were also counted and measured, respectively. Values are mean ± SE (n ≥ 5)

* Significant difference between miR156+ and WT plants at P < 0.05 (one-way ANOVA, Duncan’s test)

iThese numbers indicate how many infection threads or nodule primordia were observed out of each 10 plants