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. Author manuscript; available in PMC: 2015 Aug 17.
Published in final edited form as: Immunity. 2015 Feb 10;42(2):344–355. doi: 10.1016/j.immuni.2015.01.010

Figure 6. TIGIT Interacts with the Fap2 Protein of F. nucleatum.

Figure 6

(A) SDS PAGE gel of membrane proteins purified from the wild-type F. nucleatum 726 strain and the two mutants: D22 and K50. The Fap2 protein bands present in the wild-type F. nucleatum 726 is indicated by an arrow. M represents the protein marker. The marker molecular weights are indicated on the left.

(B) 0.5 × 106 F. nucleatum 726, the F. nucleatum mutants K50 and D22 and the CFT073 UPEC were bound in wells of ELISA plates. Plates were incubated with human TIGIT-Ig or with human DNAM1-Ig (hDNAM1-Ig, incubated only with FN726) and ELISA assays were performed. Figure shows one representative experiment out of three performed. *p < 0.002. The error bars are derived from triplicates.

(C) Outer membrane vesicle proteins extracted from the wild-type F. nucleatum 726 and from K50 were run on a 7% SDS PAGE gel, transferred to a nitrocellulose membrane and incubated with TIGIT-Ig. The molecular weights protein markers are indicated on the left.