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. 2015 Mar 16;10(3):e0118698. doi: 10.1371/journal.pone.0118698

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© 2015 Yassine et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Western blot of protein extracts from HEK cells heterologously expressed with or without plasmid containing mouse short isoform of Sun5 and revealed with anti-Sun5 antibodies 1 (Ab1). Below, gel of protein showing that both lanes were similarly loaded. (B) Similar experiment but revealed with anti-Sun5 antibodies 2 (Ab2). (C) Western blot of protein extracts from HEK cells heterologously expressed with a plasmid containing human short isoform of SUN5-DDK tagged and revealed with anti-DDK antibodies. (D) HEK cells were transfected with mouse short isoform of Sun5 and stained with Hoechst (blue, D1) and anti-Sun5 Ab1 (D2, green). D3 corresponds to overlay + phase contrast. (E) Similar experiment performed with NIH/3T3 cells.