Fig 2. Sun5 is located in the cytoplasm in pachytene spermatocyte.

(A) Pachytene spermatocyte co-stained with Hoechst (blue) and anti-Sun5 Ab1 (green) (A1). A2 corresponds to overlay + phase contrast. (B) Pachytene spermatocytes were purified by unit gravity sedimentation from spermatogenic cell suspension obtained from sexually mature males. The purity of the fraction (B1, observed with optical microscope), was assessed by counting the cells exhibiting characteristic Hoechst nuclear staining under a fluorescent microscope (B2). (C) Western blot of cytoplasmic proteins extracted from the pachytene spermatocytes showing that Anti-Sun5 Ab immunodecorates a single band around 40 KDa.