Fig 2. HMGB1 has no impact on cell surface expression of RAGE, TLR2 or TLR4.

Eosinophils isolated from peripheral blood of normal donors incubated in the presence (open bars) or absence (closed bars) of IL-5 with 0, 10 or 100 ng/ml HMGB1for 48 hours and then evaluated for the expression of (A) RAGE, (B) TLR4, and (C) TLR2. Data represent mean ± SEM of percent population of live eosinophils that are positive for indicated receptor minus staining with isotype control; n = 3 independent donors.