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. 2015 Mar 16;10(3):e0119761. doi: 10.1371/journal.pone.0119761

Fig 4. TatA does not recognize the RR motif.

Fig 4

(A) Positions of arginines in the twin-arginine motif that were mutated for experiments shown under B). (B) Analyses of cross-links to Tat components with pBpa (abbreviated “B”) at indicated twin-arginine motif positions (expression system and [pBpa] as in Fig. 1). Additional R>K exchanges are indicated. A decrease of TatBC cross-link intensities relates to decreasing affinities as indicative for the twin-arginine motif recognition (indicated at the bottom) that is confined to TatBC.