Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 17;5:9205. doi: 10.1038/srep09205

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) An increase of luciferase level in culture media was detected in the GFAP-Nanoluc-KI cell lines during directed differentiation into astrocytes. Luciferase levels shown in the bar graph were normalized by the basal level detected at day 0 in GFAP-Nanoluc-KI NSC. (b) Immunostaining showed excellent co-localization of HaloTag (red) and GFAP (green) antibodies in the GFAP-Nanoluc-KI astrocytes (D23 post differentiation). (c) Live staining of HaloTag (red) in the GFAP-Nanoluc-KI cell line before (left) and after (right) directed differentiation into astrocytes. (d) An increase of luciferase level in culture media was detected in the MAP2-Nanoluc-KI cell lines during directed differentiation into neurons. Luciferase levels shown in the bar graph were normalized by the basal level detected at day 0 in the MAP2-Nanoluc-KI NSC. (e) Immunostaining showed excellent co-localization of HaloTag (red) and MAP2 (green) antibodies in the MAP2-Nanoluc-KI neurons (D18 post differentiation). (f) Live staining of HaloTag (red) in the MAP2-Nanoluc-KI cell line before (left) and after (right) directed differentiation into neurons. (g) A series dilution of GFAP-Nanoluc-KI (left) and MAP2-Nanoluc-KI (right) iPSC were plated and luciferase level from the media was tested. The minimum cell amount needed was 10 K for detectable luciferase level of both GFAP-Nanoluc-KI and MAP2-Nanoluc-KI iPSC lines. Scale Bars shown in this Fig are all 100 μm.