Figure 2. Muscle pathology in WT and SHP-1^−/− mice.

12 day old me^v/me^v mice (bottom) and WT littermates (top) were infected with 6×10⁵ PFU TMEV or mock supernatant and sacrificed at 9 days P.I. (A) Representative images of dissected mice; arrows indicate observed muscle disease by apparent calcification. (B) Alizarin red (left) and H&E (middle) stains were performed on serial skeletal muscle sections; arrows point to corresponding calcified fibers in adjacent sections. Scale bars represent 50µm, n=3. (B, right) Muscle sections were stained with picrosirius red, n=4, scale bars depict 50µm.