Figure 6. Gene expression in muscle or CNS-infiltrating cells.

(A) CD45⁺CD11b⁺Ly6c⁺ cells were sorted from skeletal muscle of WT and me/me littermates at 5 days P.I. (B) CD45⁺ cells were sorted from spinal cords of animals shown in (A). (A–B) RNA levels of IFN-β and M1/M2-associated genes were measured using a multiplex bead-based assay. Gene expression levels were normalized to the mean of 3 housekeeping genes (HPRT1, TBP1 and GAPDH) for each sample; n=3.