Figure 3.

Global comparisons of transcript profiles and DEGs in response to GA and ABA treatments. Petals of inflorescences at stage 3 were detached, placed on filter papers soaked with 3% sucrose (Control) or with hormone supplementation and were cultured as described in the text. Basal regions of the petals were collected after 2 or 12 h of culture, followed by total RNA extraction for the subsequent RNA-Seq analyses and data mining. (A) Global transcript profiles at the 2 or 12 h time point after culturing with hormone supplementation as indicated. Each box plot shows the distribution of the relative transcription level [log₂(fold-change)] of genes with at least one read mapped to the G. hybrida transcriptome in one sample. The red line indicates a one-fold change relative to the control transcription level. (B) Venn diagram for DEGs identified at each time point of hormone application as indicated. (C) Number of DEGs antagonistically regulated by GA and ABA. Class I and III correspond to the DEGs after 2 and 12 h of hormone application, respectively, and Class II was identified by comparing 12 h samples with 2 h samples.