Figure 5. Ciliary ectosomes possess agglutinin receptor binding and signaling activity.
(A) TEM of cilia on minus vegetative cells (left panel) and gametes (right panel) that had been mixed with ciliary ectosomes. Only the cilia on the gametes bound ciliary ectosomes. (B) Immunoblot analysis of binding of SAG1-C65-HA-containing ectosomes to whole cells, cell bodies, and cilia of minus gametes. The initial sample of cells + ectosomes is also shown (input).The lanes contain equal cell equivalents. (C) Cilium-generated signaling was activated when ciliary ectosomes were mixed with HAP2-HA minus gametes, as assessed by activation-induced upregulation of expression of the gamete fusion protein, HAP2.