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. 2015 Feb 17;125(3):1269–1285. doi: 10.1172/JCI76452

Figure 4. M2 macrophages initiate a WNT-high state in CC.

Figure 4

(A) Immunohistochemistry in TAA-induced CC for WNT7B (green) and CD68 (red). White arrows, macrophage expression of WNT7B; yellow arrows, epithelial positivity for WNT7B. (B) Schematic representing the GFP BM transplant (Tx.) strategy. (C) Immunohistochemistry for CD68 or CD163 (red) and GFP (green) in 26-week TAA BM-transplanted rats. Dotted line, tumor boundary. (D) Immunohistochemistry of GFP (green)/CD68 (red) dual-positive TAMs expressing CD206 (white). (E) Quantification of CD68/GFP dual-positive TAMs expressing CD206 (n = 10). (F) Immunohistochemistry for GFP (green), CD68 (white), and WNT7B (red) in 26-week TAA CC. (G) Quantification of CD68-positive macrophages expressing WNT7B in rat CC (n = 10). Data are presented as mean ± SEM. Photomicrograph scale bars: 50 μm; insets, 10 μm.