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. 2015 Feb 17;125(3):1269–1285. doi: 10.1172/JCI76452

Figure 6. Macrophage inhibition reduces CC growth in vivo.

Figure 6

(A) Schematic representing macrophage depletion in TAA using Lipclod. (B) mRNA expression of Wnt7b, Wnt10a, and Porcn following macrophage depletion in TAA CC using vehicle versus Lipclod (n = 5 vs. n = 7). (C) Immunohistochemistry on vehicle- versus Lipclod-treated TAA tissue for WNT7B (red) and KRT19 (green). (D) mRNA expression of WNT pathway target genes following Lipclod depletion of macrophages (n = 6 per group); green denotes downregulation. Represented as a 3-fold change; P < 0.05 (E) H&E of rat liver from TAA rats treated with vehicle versus Lipclod. (F) Quantification of area and number of rat TAA-induced CC tumors following treatment with vehicle or Lipclod (n = 14 vs. n = 5). Data are represented as mean ± SEM. Mann-Whitney U test; *P < 0.05, **P < 0.01. Scale bars in C: 50 μm; insets, 10 μm. Scale bars in E: 1 mm.