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. 2015 Feb 17;125(3):1269–1285. doi: 10.1172/JCI76452

Figure 7. Inhibition of the canonical WNT signaling pathway alters cell viability in vitro.

Figure 7

(A) MTT assay using SNU-1079, CC-SW-1, SNU-1196, CC-LP-1, and WITT-1 CC lines treated with increasing concentrations of ICG-001 or C-59. Dose response data are presented as the mean of 3 experimental replicates that were run in triplicate. (B) Quantification of caspase-3/7 activity and BrdU incorporation with increasing concentrations of ICG-001 in SNU-1079, CC-SW-1, SNU-1196, CC-LP-1, and WITT-1 CC lines. (C) Quantification of caspase-3/7 activity (left graph) and BrdU incorporation (right graph) with increasing concentrations of C-59 in SNU-1079, CC-SW-1, SNU-1196, CC-LP-1, and WITT-1 CC lines. (D) mRNA expression of WNT target genes and cell cycle genes in CC-LP-1 and SNU-1079 cells following inhibition with ICG-001. Data are presented as >2-fold-change. (E) mRNA expression of WNT target genes and cell cycle genes in CC-LP-1 and SNU-1079 cells following inhibition with C-59. Data are presented >2-fold-change. Dose response data are presented as the mean of 3 experimental replicates that were run in triplicate. Kruskal-Wallis test; **P < 0.01, ***P < 0.001. Gene expression data represent pooled material from 3 experimental replicates run in triplicate.