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. 2015 Feb 9;125(3):1215–1227. doi: 10.1172/JCI76693

Figure 4. Serum-derived exosomes from healthy controls and patients with IBD.

Figure 4

(A and B) Immunoblots of ANXA1, CD9, and ANXA5 in isolated exosomes from sera of human (A) healthy controls and (B) patients with IBD. Exosomes were precipitated from human pooled sera using SBI’s Serum ExoQuick precipitation reagent (data are representative of n = 3 experiments). (C) Representative side scatter versus bright-field area (SSC/Ch12) and Ch04 fluorescence plots for EV isolates acquired with 1-μm and 500-nm reference beads (100-nm and 50-nm beads were acquired separately and overlaid) of EVs isolated from human sera by ultracentrifugation (n = 3). (D) Representative bright-field (Ch01), Ch04, and Ch012 images of each population of beads and EVs (n = 3) (original magnification, ×60). Scale bar: 7 μm. (E) EVs were stained with BODIPY-Texas Red and conjugated fluorescent antibodies against ANXA1 (n = 3). Data are expressed as mean ± SD. *P = 0.0194. Statistical comparisons were performed by 2-tailed Student’s t test.