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. 2015 Feb 2;125(3):1129–1146. doi: 10.1172/JCI78280

Figure 1. Hierarchy of protective CD8 T cell responses and Ag expression after rAd vaccination.

Figure 1

(A) Frequency of tetramer+ CD8+ T cells in spleens at peak (day 23) or once memory CD8+ T cell responses were established (day 70) after vaccination with human-derived rAds. (B) Bacterial load in spleens at day 108 after challenge with L. monocytogenes expressing Gag. (C) Ag transcript expression in dLNs after vaccination with human-derived rAds at 1 × 109 PU or 1 × 108 PU. (D) Tetramer+ CD8+ T cell responses at peak or memory, (E) bacterial load, and (F) Ag expression for chimpanzee-derived rAds. (G) Tetramer+ CD8+ T cell responses at peak or once memory CD8+ T cell responses were established, (H) bacterial load, and (I) Ag expression for simian-derived rAds. For CD8 T cell responses and bacterial load analysis, mice received all rAds at 1 × 108 PU; rAd35 was administered at both 1 × 109 PU (rAd35hi) and 1 × 108 PU (rAd35lo). For Ag expression, mice received rAds at 1 × 109 PU or 1 × 108 PU as indicated. Error bars indicate mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, Mann-Whitney test. Dashed lines indicate the limit of detection (LOD). Data represent at least 2 independent experiments (n = 3–6).