Figure 7. Module analysis over time after rAd vaccination.

(A) Radar plots comparing the kinetics of innate gene activation: either upregulation or downregulation of each module or individual genes from modules C2 and C3 relative to the PBS control with each rAd at 8, 24, and 72 hours after vaccination. Axes represent (top) maximum = +4, minimum = –4, circles = +4 (outside), +3, +2, +1, 0 (gray circle), –1, –2, –3, and –4 or (bottom) maximum = +8, minimum = –1, circles = +8 (outside), +7, +6, +5, +4, +3, +2, +1, 0 (gray circle), and –1. (B and C) Amount detected in serum of (B) IFN-α at 10 hours and (C) IP-10 at 24 hours after vaccination with rAds. On radar plots, each plot partition represents ± 1 log₂ fold change relative to PBS. Error bars represent mean ± SEM. *P ≤ 0.05, for each rAd compared with PBS, Mann-Whitney test. Data in B and C represent 2 independent experiments (n = 4–6).