Skip to main content
. 2015 Feb 9;125(3):1228–1242. doi: 10.1172/JCI77656

Figure 10. Ep3a/b variants mediate membrane localization of MT1-MMP through RhoA/ROCK-dependent cytoskeletal remodeling.

Figure 10

(A) Rhodamine phalloidin staining of F-actin in Ep3–/– and WT PASMCs after normoxia or hypoxia exposure. (B) Effect of LB, C3, and Y-27632 on F-actin polymerization in Ep3-­deficient PASMCs with forced expression of Ep3a or Ep3b. (C) Quantification of F-actin/G-actin ratio in hypoxia-treated, Ep3a/b-complemented PASMCs in the presence or absence of LB, C3, or Y-27632 (n = 3). *P < 0.05 versus vector and #P < 0.05 versus vehicle by 2-way ANOVA with Bonferroni’s post-hoc analysis. (D) Immunoblot analysis of MT1-MMP membrane expression in Ep3a/b-overexpressing PASMCs with or without pretreatment with LB, C3, or Y-27632. (E–G) Immunoblot analyses of p-SMAD2, fibronectin, and collagen I in Ep3a/b-complemented PASMCs with or without pretreatment with LB, C3, or Y-27632.